Characterization of non-Tg, Tg and Tg/Tg cortical neuron-enriched cultures.
A) Western blot and graph showing UOx expression in wild-type (non-Tg) and UOx-expressing neurons (Tg and Tg/Tg) normalized to the β-actin level. Note that UOx was not detected in wild-type neurons (n = 3). B) Effect of UOx expression on intracellular urate content in neurons normalized to the protein level (n = 3). C) UOx activity in the media of non-Tg and UOx-expressing neurons (n = 6). Student's t test: ##P = 0.005 vs Tg value; one-way ANOVA followed by Newman-Keuls test: **P<0.01, ***P<0.001 vs non-Tg value and ###P<0.001 vs Tg value.