Changes in the proteomes of the PHA-negative mutant <i>R. eutropha</i> PHB<sup>-</sup>4 compared to the wild type <i>R. eutropha</i> H16.

<p>Cells were cultivated in MSM medium under conditions promoting PHB synthesis. Extracted proteins were focused using pH 5 to 8 nonlinear strips. Dual channel images were generated by employing the Delta2D software. (A) Cells of the exponential growth phase. Wild type H16, blue spots; mutant PHB<sup>-</sup>4, orange spots. (B) Cells of the stationary growth phase. Wild type H16, blue spots; mutant PHB<sup>-</sup>4, orange spots. Arrows and numbers mark proteins with significantly different levels of expression in comparison to those of wild type H16 and mutant PHB<sup>-</sup>4. Detailed information about the detected proteins is compiled in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0095907#pone-0095907-t002" target="_blank">Table 2</a> and Fig. 3.</p>