Changes in the proteomes of the PHA-negative mutant R. eutropha PHB^-4 compared to the wild type R. eutropha H16.

Cells were cultivated in MSM medium under conditions promoting PHB synthesis. Extracted proteins were focused using pH 5 to 8 nonlinear strips. Dual channel images were generated by employing the Delta2D software. (A) Cells of the exponential growth phase. Wild type H16, blue spots; mutant PHB^-4, orange spots. (B) Cells of the stationary growth phase. Wild type H16, blue spots; mutant PHB^-4, orange spots. Arrows and numbers mark proteins with significantly different levels of expression in comparison to those of wild type H16 and mutant PHB^-4. Detailed information about the detected proteins is compiled in Table 2 and Fig. 3.