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Cellular immune responses.
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posted on 2013-02-20, 21:15 authored by Jae-Min Song, Bao-Zhong Wang, Kyoung-Mi Park, Nico Van Rooijen, Fu-Shi Quan, Min-Chul Kim, Hyun-Tak Jin, Andrew Pekosz, Richard W. Compans, Sang-Moo KangThe cellular immune responses were assessed with splenocytes isolated from mice 4 weeks boost immunization with M2 VLPs or M1 only VLPs without M2 (Mock) before challenge (A–C) and at 4 day post challenge (D–F) with A/Philippines/82 virus (n = 6). Cells from spleen (A, D), lung (B, E), and BAL (C, F) were stimulated with M2 peptides for 2 days and cytokine forming cell spots were determined by ELISPOT assay. The asterisk indicates a significant difference between M2VLP and Mock groups, * p<0.05, ** p<0.01.
