Cellular cytotoxicity and <i>P. aeruginosa</i> adhesion and invasion in response to simvastatin treatment.

<p>Cells were treated with 10 µM simvastatin for 24 hours (<b>A, B, C</b>), following which they were infected with <i>P. aeruginosa</i> PAO1at MOI 50∶1 (<b>A, C</b>). (<b>A</b>) Simvastatin significantly induced cytotoxicity but did not alter <i>P. aeruginosa</i>-mediated LDH release at 6 hours post infection. (<b>B</b>) Simvastatin-induced cytotoxicity was not significantly altered in si-wtKLF6 knockdown cells demonstrating wtKLF6 was not implicated in simvastatin-mediated cytotoxicity of lung epithelial cells. (<b>C</b>) Simvastatin treatment increased <i>P. aeruginosa</i> adhesion to cells, but (<b>D</b>) did not influence invasion at 3 hours of infection. *<i>P</i>, ≤0.05, **<i>P</i>, ≤0.01, *** <i>P</i>, ≤0.001.</p>