Cell characterization after long term growth on Synthemax II microcarriers in xeno-free medium.

(A) Fluorescent image of hMSCs on Synthemax II microcarriers after calcein AM staining. (B) Phase image of hMSCs after migration off Synthemax II microcarriers to a 6-well plate. (C) hMSC karyotype analysis after 7 passages on Synthemax II microcarriers in stemgro hMSC medium. (D) After 5 passages on Synthemax II microcarriers, hMSCs were induced to differentiate into adipogenic, osteogenic, and chondrogenic lineages and were stained with oil red O, alizarin red, and alcian blue, respectively. Representative images of stained cells from both media are shown.