Cell adhesion on immobilized E1-5 and E1-2 fusion constructs.
(A) Upper row: fluorescence image of EcadEGFP/L-cells on microcontact printed surfaces functionalized with E1-5; lower row in presence of 2 μl/ml blocking antibody (DECMA). Green: EcadEGFP fluorescence, red: immunostaining against E-cadherin, merge: overlay with nuclei staining (DAPI). (B) Statistical analysis of spread EcadEGFP/L-cells on E1-5 pattern (black) or on EG4-thiol (white) without treatment or in the presence of 10 mM EDTA or 2 μl/ml DECMA. Spread cells as percentage of total attached cells per substrate type (mean±SE). N: numbers of experiments, n: numbers of cells. (C) Statistical analysis of spread EcadEGFP/L-cells on E1-2 pattern (black) or on EG4-thiol (white). N: numbers of experiments, n: numbers of cells, mean±SE. (D) Statistical analysis of EcadEGFP/cells on E1-5 pattern of different BGT:MT ratios as indicated (mean±SE). The dilutions used to create varying distances of E-cadherin monomers and the corresponding theoretical intermolecular distances are indicated below.