Cav-1 immunohistochemical staining and protein content of wild type mice PCLS and E10.

(A) Immunohistochemical staining of Cav-1 of representative untreated lung slices, slices treated with 1600 µg/ml silica and slices treated with 1600 µg/ml silica and co-cultured with AMJ2-C11 macrophages as well as Western Blot analysis of PCLS. Data was normalized to the control. PCLS was incubated for 72 hours. (B) Immunoblots of E10 experiments without and with AMJ2-C11 macrophages. For E10 experiments nano-silica concentrations ranging from 50 to 200 µg/cm2 flask area and an incubation time of 48 hours were used. Each silica concentration was normalized to the control. γ-Tub is the loading control, n = 6–7. Scale bar = 50 µm.