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CTC Flow cell operation and performance.
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posted on 2014-01-29, 03:42 authored by Jessamine P. Winer-Jones, Behrad Vahidi, Norma Arquilevich, Cong Fang, Samuel Ferguson, Darren Harkins, Cory Hill, Erich Klem, Paul C. Pagano, Chrissy Peasley, Juan Romero, Robert Shartle, Robert C. Vasko, William M. Strauss, Paul W. Dempsey(A) The LiquidBiopsy CTC flow cell. (B) A cartoon illustrating how target cells are pulled from the sheath flow while non targets move through the flow cell unhindered. (C) Efficiency of recovery of target cells using EpCAM based recovery: Incremental numbers of MCF7 (N = 34), HCC1419 (N = 27) or A549 (N = 65) cells were spiked into NHD blood and purified on the CTC flow cell. Recovered cells were enumerated in the flow cell. Graphs show number of targets spiked per mL of blood against the yield +/−1 SD from an average of between 7 and 27 experiments.
