CRTR-1 binds the CP2 response element and forms DNA-binding complexes with other CP2 family members.
A. Nuclear extracts prepared from untransfected or pEF-CRTR-1 transfected HEK293T cells were incubated with FAM-labelled DNA probe containing a wild type (WT) or mutated (Mut) CP2 response element. Binding reactions were performed in the presence or absence of anti-CRTR-1 antibody or pre-immune serum (pre-immune) as indicated. Competitions were performed in the presence of 50-, 100-, or 200 fold excess unlabelled competitor oligo containing the wild type or mutated CP2 response element. B. Nuclear extracts prepared from HEK293T over-expressing CRTR-1 and the indicated CP2 family member were incubated with FAM-labelled DNA probe containing a wild type α-globin CP2 response element in the presence or absence of anti-CRTR-1 antibody. Probe was in excess in all samples. Protein-DNA complexes were resolved by electrophoresis on a 4% native polyacrylamide gel. SC, specific complex; SS, super-shifted complex; NS, non-specific complex.