Figure_2.tif (1.46 MB)
CDK1 activity is increased after spinal cord injury.
figure
posted on 2013-02-20, 01:57 authored by Junfang Wu, Giorgi Kharebava, Chunshu Piao, Bogdan A. Stoica, Michael Dinizo, Boris Sabirzhanov, Marie Hanscom, Kelsey Guanciale, Alan I. FadenWestern blotting analysis of common CDK substrates, CDK1 co-activator cyclin B1 and phosphorylations of specific CDK1 substrate (Ser54)-n-myc was performed in homogenates obtained from intact and injured spinal cord. A. Cyclin B1 expression was upregulated at all time points tested. Phosphorylation (Ser54) of n-myc and phospho-CDK substrate motif signal levels were increased from 5 h to day 7. B–D. Quantification of respective western blots in panel A. n = 4 rats/time point. *P<0.05 vs sham group.
