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Both endogenous and exogenous Osx are increased by proteasome inhibitor treatment.

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posted on 2013-02-19, 15:15 authored by Yanyan Peng, Kaikai Shi, Lintao Wang, Jianlei Lu, Hongwei Li, Shiyang Pan, Changyan Ma

A. Saos-2 cells were treated with MG-132 (20 µM) for various periods of time. Endogenous Osx protein was detected by Western blot analysis with an anti-Osx antibody. B. HEK 293T cells were transfected with Flag-hOsx plasmids and then treated with MG-132 (20 µM) for various periods of time. Exogenous Osx protein was detected by Western blot analysis with an anti-Flag antibody. C. Saos-2 cells were treated with lactacystin (10 µM) for various periods of time. Endogenous Osx protein was detected by Western blot analysis with an anti-Osx antibody. D. HEK 293T cells were transfected with Flag-hOsx plasmids and then treated with lactacystin (10 µM) for various periods of time. Exogenous Osx protein was detected by Western blot analysis with an anti-Flag antibody. β-actin served as a loading control. Results are shown for one of three independent experiments.

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