Biodegradation of (A) ANT and (B) BaA by the recombinant laccases and their LMSs.

The reaction was performed in 1×McIlvanie buffer (pH 4) at 30°C by using 5 µg of enzyme with or without 1 mM HBT or TEMPO. Residual amount of PAHs was resolved by high-performance liquid chromatography (Waters) equipped with a C-18 reverse-phase column (4.6 mm×250 mm, Grace) at ambient temperature and quantified by a photodiode array detector at 252 nm. Results shown are the average of three independent experiments ± S.D.