Biochemical characterization of S<sup>209</sup>, C<sup>33</sup> and L<sup>399</sup> CsACS2 isoforms.

<p>(A–C) Initial velocity measured at 50 µM of PLP. Experimental data (circle) were fit into a line following Michaelis-Menten equation. The resulting parameters are indicated in the table (insert). (D) Enzymatic activity of CsACS2 (black bars), S<sup>209</sup> (orange bars), C<sup>33</sup> (red bars) and L<sup>399</sup> (magenta bars) protein forms. Specific activities were measured on dialyzed enzymes in the presence of 60 µM SAM and various PLP concentrations.</p>