Biochemical characterization of S209, C33 and L399 CsACS2 isoforms.
(A–C) Initial velocity measured at 50 µM of PLP. Experimental data (circle) were fit into a line following Michaelis-Menten equation. The resulting parameters are indicated in the table (insert). (D) Enzymatic activity of CsACS2 (black bars), S209 (orange bars), C33 (red bars) and L399 (magenta bars) protein forms. Specific activities were measured on dialyzed enzymes in the presence of 60 µM SAM and various PLP concentrations.