Binding of Sp2 deletion mutants to selected target promoters.

<p>(A) Schematic representation of Sp2 deletion mutants expressed in <i>Sp2ko</i> MEFs. (B) Western blot analysis (anti-Flag) of the Sp2 mutants shown in panel A. (C) Subcellular localization of the Sp2 mutants in rescued <i>Sp2ko</i> MEFs. Sp2 was detected by immunofluorescence using anti-Flag antibodies. Nuclei were counterstained with DAPI. (D) ChIP-qPCR analysis of Sp2 mutants at selected target promoters (<i>Sp2</i>, <i>Osbp</i> and <i>Amd1</i>). The <i>Sp2–2</i>.<i>5</i> kb region served as a negative control. The percent of input values are mean +/- SD (n = 3).</p>