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Binding of Sp2 deletion mutants to selected target promoters.

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posted on 2015-03-20, 04:02 authored by Sara Völkel, Bastian Stielow, Florian Finkernagel, Thorsten Stiewe, Andrea Nist, Guntram Suske

(A) Schematic representation of Sp2 deletion mutants expressed in Sp2ko MEFs. (B) Western blot analysis (anti-Flag) of the Sp2 mutants shown in panel A. (C) Subcellular localization of the Sp2 mutants in rescued Sp2ko MEFs. Sp2 was detected by immunofluorescence using anti-Flag antibodies. Nuclei were counterstained with DAPI. (D) ChIP-qPCR analysis of Sp2 mutants at selected target promoters (Sp2, Osbp and Amd1). The Sp2–2.5 kb region served as a negative control. The percent of input values are mean +/- SD (n = 3).

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    PLOS Genetics

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    genomic binding patterngenomic binding site selectionccaatgcSp 3 mutantsSp 3 Transcription factorsgenomic binding patternsSp 3Sp 2binding site selectionmefSp 2 potentiates bindingSp 2 Potentiates Recruitment

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