BNIP-H co-localizes with Pin1 in differentiating PC12 and P19 cells.
PC12 (A) and P19 cells (B) were fixed, permeabilized and probed with the indicated antibodies, followed by appropriate fluorophore-conjugated secondary antibodies and analyzed by confocal microscopy. PC12 cells were treated with NGF for 24 and 48 hours, respectively (panel I). Undifferentiated PC12 cells grown for 48 hours were used in panel II. P19 cells were treated with retinoic acid for five days and allowed to differentiate for another six days without retinoic acid. Scale bar in (A) 20 µm for panel I, and 10 µm for panel II, in (B) 10 µm.