BAG1 protein expression in a cohort of leukemia patients and leukemic cell lines.

A) Western Blot (WB) analysis for the study of the expression of three major BAG1 protein isoforms in total protein extracts, and expression of BAG1in CD34+ and CD34- cell fractions sorted from HBM. HL60 cell line is used as positive control B) WB analysis of the expression of BAG1 isoforms in total protein extracts and BAG1L/-1M expression in nuclear proteins isolated from four AML samples at diagnosis (Diag) and from human AML cell lines (C). β-ACTIN is shown as total and HDAC1 as nuclear proteins loading control.