Association of miR-124 to hStau1 complexes in undifferentiated and differentiated neuroblastoma cells.
2014-11-25T03:06:46Z (GMT) by
<p>Cultures of SH-SY5Y neuroblastoma cells were differentiated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113704#s2" target="_blank">Materials and Methods</a>. Total cell extracts were isolated from cells prior to differentiation (day 0) or at a final stage of differentiation (day 10) and fractionated on a Sephacryl S-400 column. (A) The various fractions were analysed by Western-blot with antibodies specific for Ago2 and hStau1 The gels to analyse hStau1 were run longer than in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113704#pone-0113704-g004" target="_blank">Fig. 4</a> to better separate hStau1 55 and 63 kDa isoforms. (B) RNA was isolated from the fraction pools indicated and the concentration of miR-124 was determined by TaqMan RT-qPCR. (<b>C</b>) The amounts of hStau1<sup>55</sup> and hStau1<sup>63</sup> isoforms were determined by Western-blot (see A) and their ratios is presented as average and standard deviations of 3 determinations. <b>*</b> indicates a p-value<0.05 in a two-tailed Student's t-test.</p>