Assessment of integrin β3 amount and result of trypsin-digestion adhesion assay.
A) Relative mRNA amount of integrin β3 in different cell lines before CSFV infection evaluated through two-step qPCR. ITGB3 denotes integrin β3. *** marked above ST column denotes extremely significant difference between ST and EC, IEC or PK. B) Western blot analysis of the amount of integrin β3 expressed in four cell lines. GAPDH denoting the house keeping gene of Glyceraldehyde 3-phosphate dehydrogenase, were used as the loading control. Ratio of the signal intensity was calculated through NIH ImageJ. C) Relative mRNA amount of integrin β3 in different cell lines after CSFV infection, compared to nc group. “nc” denotes the cell without CSFV infection. (***p<0.001, extremely significant difference; **p<0.01, very significant difference; *p<0.05 significant difference, n.s., no significant difference). D) Results of trypsin-digestion assay for cell adhesion. Cells were pictured after the incubation with 0.25% trypsin solution at 37 °C for 2 minutes. Cells with round edge indicated that they were digesting off from the ECM.