Assessment of integrin β3 amount and result of trypsin-digestion adhesion assay.

<p><b>A)</b> Relative mRNA amount of integrin β3 in different cell lines before CSFV infection evaluated through two-step qPCR. ITGB3 denotes integrin β3. *** marked above ST column denotes extremely significant difference between ST and EC, IEC or PK. <b>B)</b> Western blot analysis of the amount of integrin β3 expressed in four cell lines. GAPDH denoting the house keeping gene of Glyceraldehyde 3-phosphate dehydrogenase, were used as the loading control. Ratio of the signal intensity was calculated through NIH ImageJ. <b>C)</b> Relative mRNA amount of integrin β3 in different cell lines after CSFV infection, compared to nc group. “nc” denotes the cell without CSFV infection. (***p<0.001, extremely significant difference; **p<0.01, very significant difference; *p<0.05 significant difference, n.s., no significant difference). <b>D)</b> Results of trypsin-digestion assay for cell adhesion. Cells were pictured after the incubation with 0.25% trypsin solution at 37 °C for 2 minutes. Cells with round edge indicated that they were digesting off from the ECM.</p>