Fig_4.tif (2.15 MB)
Arginine-containing peptides from cells grown in 13C6-arginine (Arg-6) exhibit a single lower-molecular-mass peak that indicates loss of a single “heavy” atom from a portion of the Arg-6 pool.
figure
posted on 2015-06-15, 02:55 authored by Weronika E. Borek, Juan Zou, Juri Rappsilber, Kenneth E. SawinMass spectra of tryptic peptides (SLAELCLGLVQEAIDASILSQQESSNSLDLVR, LNQVELQLSER, and IWHHTFYNELR, in (A), (B) and (C), respectively) from S. pombe (A, B) Mto2[17A]-GFP and (C) actin, isolated from car2∆ arg1-230 lys3-37 cells grown in either (i) unlabeled arginine (Arg-0) and unlabeled lysine (Lys-0), or (ii) 13C6-arginine (Arg-6) and 13C615N2-lysine (Lys-8), as indicated. In peptides from cells grown in Arg-6, the single “pre-peak” (indicated in blue) indicates conversion of a portion of Arg-6 to 13C5-arginine (Arg-5). Masses of monoisotopic peaks for each growth condition (i.e., assuming no conversion of labeled arginine) and inferred peptide charge-states are indicated in magenta.