<i>Apoe</i> expression is reduced in the parietal cortex and CA1 of aged mice.

<p>(A) Representative sections of the FPC from young and aged brains hybridized with an <i>Apoe</i> riboprobe. (B) Quantification of <i>Apoe</i> hybridized area in the cortex shows a significant decrease of <i>Apoe</i> expression in the aged mice. (C) Representative sections of the hippocampal CA1 region from young and aged brains hybridized with an <i>Apoe</i> riboprobe. (D) <i>Apoe</i> hybridized area in the CA1 shows a significant decrease of <i>Apoe</i> expression in the aged mice. (E) Representative sections of the CC from young and aged brains hybridized with <i>Apoe</i> riboprobe. (F) A significant increase of <i>Apoe</i> hybridized area is measured on the aged CC. (G) Representative images of <i>Apoe</i> in situ hybridization in the cortex and CC showing decreased expression of <i>Apoe</i> in the cortex and increased expression in the CC in aged mice compared with young mice. (H) No differences in APOE protein levels were found by western blotting of whole brains from young and aged mice. Values in (B, D, and F) are relative mean <u>+</u> SEM to the young values, <i>n</i> = 6 per group. In (D) **<i>p</i> < 0.001 and in (F) *<i>p</i> = 0.0364 by unpaired <i>t</i> test. Scale Bars: 600 μm (A), 50 μm (C), 100 μm (E and G). The data used to make this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002279#pbio.1002279.s001" target="_blank">S1 Dataset</a>.</p>