Aortic composition in HFD rats.

<p>Aortas from rats fed a standard diet (3.5% fat) or a high fat diet (HFD, 33.5% fat) were analyzed. Representative pictures of slides stained for collagen and elastin are presented (magnification 40X) (<b>A</b>). mRNA expression of collagen type I, fibronectin, TGF-β, CTGF in aorta from controls and HFD rats (<b>B</b>). Protein expression of collagen type I, elastin, MMP-2, TIMP-2, TGF-β and CTGF in aorta from controls and HFD rats (<b>C</b>). All conditions were performed at least by triplicate. Scale bar 50 µm. Histogram bars represent the mean ± SEM of 6–7 animals, in arbitrary units normalized to HPRT and β-actin for cDNA and protein respectively. *p<0.05; ***p<0.001 <i>vs</i>. control group.</p>

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hfd

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