Anticancer (IC₅₀) and hemolytic activities (MHC) of peptides against cancer cells and human red blood cells.

^aAnticancer activity (IC₅₀) represents the concentration of peptides at which cell viability was inhibited by 50% in comparison with the untreated cells. The MTT assay was repeated in triplicate, and IC₅₀ value was determined by averaging three repeated experiments.

^bGM of the anticancer activity (IC₅₀) for the four cancer cell lines.

^cHemolytic activity (MHC) was determined using human red blood cells after incubation with peptides for 1 h. If no hemolytic activity was observed at 500 μM, a value of 1000 μM was used for calculating the therapeutic index.

^dTherapeutic index = MHC/IC₅₀. Larger values indicate greater anticancer specificity.

GM, geometric mean; MHC, minimal hemolytic concentration.

Anticancer (IC₅₀) and hemolytic activities (MHC) of peptides against cancer cells and human red blood cells.