Antibody binding kinetic constants to selected MPER probes.

2012-07-19T02:07:19Z (GMT) by Javier Guenaga Richard T. Wyatt
a<p><b>MPER peptide</b>: EQELL<b><u>ELDKWA</u></b>SLWNWFDITKWLWYIKKKKGSKKK.</p>b<p><b>Linked peptide</b>: EQELL<b><u>ELDKWA</u></b>SL<u>GGGGSGG</u>WNWFDITKWLWYIKKKKGSKKK.</p>c<p><b>ES2 scaffold</b>: scaffold protein displaying the 2F5 gp41 epitope stabilized in the antibody-bound conformation.</p>d<p>Association rate (k<sub>on)</sub> is measured in (1/Ms 10<sup>5</sup>).</p>e<p>Dissociation rate (k<sub>off</sub>) is measured in (1/s 10<sup>−3</sup>).</p>f<p>Affinity constant (KD) is measured in (M 10<sup>−9</sup> or nM).</p>