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Anti-apoptotic effects of fibroblasts from the hHO-1-Tg pig.

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posted on 2013-02-19, 22:09 authored by Hye-Jung Yeom, Ok Jae Koo, Jaeseok Yang, Bumrae Cho, Jong-Ik Hwang, Sol Ji Park, Sunghoon Hurh, Hwajung Kim, Eun Mi Lee, Han Ro, Jung Taek Kang, Su Jin Kim, Jae-Kyung Won, Philip J. O'Connell, Hyunil Kim, Charles D. Surh, Byeong-Chun Lee, Curie Ahn

A: Cell viability was measured using the CCK-8. After induction with hTNF-α (25 ng/ml) and CHX (10 µg/ml) for 15 hours, the hHO-1 group had a lower percentage of viable cells. B and C: FACS analysis of hTNF-α and CHX-mediated apoptosis in fibroblasts. Apoptosis was analyzed using annexin V staining. The numbers indicate the proportion of apoptotic cells in the NPCCs after 15 hr incubation with hTNF-α and CHX. D: Activities of caspase 3/7 were measured in fibroblasts. Human HO-1 protected transgenic fibroblasts from apoptosis after 15 hr of incubation with hTNF-α and CHX. After Zn(II)PPIX treatment, Ad-hHO-1 increased activity of caspase 3/7. Each experiment was repeated three times. (* p<0.05, ** p<0.01, *** p<0.001, NT: no treatment).

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