Anti-4-1BB pre-activated PDCA-1<sup>+</sup> B cells inhibit in vitro Ig class switching.

<p><b>A</b> PDCA-1<sup>+</sup> B cells were sort-purified from spleens of naïve B6 mice and stained as indicated, and flow cytometry was performed. One of four independent experiments is shown (<i>n</i> = 4). <b>B</b> Purified splenic PDCA-1<sup>+</sup> B cells were stimulated with the indicated agonists, after which in vitro Ig class switching was analyzed by flow cytometry. One of four independent experiments is shown (<i>n</i> = 4). <b>C</b> Purified splenic PDCA-1<sup>+</sup> B cells were stimulated with soluble anti-4-1BB (5 µg/ml) and/or IL-7 (100 ng/ml) for 3d then re-stimulated with LPS/IL-4 for 4d, after which in vitro Ig class switching was analyzed by flow cytometry. One of four independent experiments is shown (<i>n</i> = 4).</p>

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