Anatabine reduces hyperactivity and disinhibition in 10.5 month-old Tg PS1/APPswe mice.

10.5 month-old Tg PS1/APPswe and their control littermates tested in EPM after treatment duration of 0.5 months with anatabine. A) Representative path tracks in the closed and open arms of the elevated plus maze by 10.5 months old Tg PS1/APPswe and their control wild-type littermate receiving regular drinking water (placebo) and anatabine at a dosage of 10 and 20 mg/Kg/Day in their drinking water are shown. B and C) The histogram represents the total distance travelled and mean velocity of Tg PS1/APPswe mice and their control wild-type littermates receiving regular drinking water (placebo) and anatabine at a dosage of 10 or 20 mg/Kg/Day. ANOVA revealed a significant main effect of the genotype for the total distance travelled (P<0.009) and mean velocity (P<0.036). Post-hoc analyses show that Tg PS1/APPswe placebo mice travelled more distance compared to wild-type placebo (P<0.017) and showed greater velocity compared to their control wild-type placebo mice (P<0.036). A significant reduction in hyperactivity was observed in Tg PS1/APPswe mice receiving anatabine at a dosage of 20 mg/Kg/Day compared to Tg PS1/APPswe mice receiving regular drinking water (placebo) (P<0.001). D) The histogram represents the average amount of time spent by Tg PS1/APPswe and wild-type mice in the open arms of the elevated plus maze. ANOVA reveals a significant main effect of the genotype (P<0.002) as well as an interactive term between genotype and anatabine (P<0.018) for the time spent in the open arm. Post-hoc comparisons show that Tg PS1/APPswe placebo mice spent significantly more time in the open arm than wild-type control mice (P<0.001). Tg PS1/APPswe mice receiving anatabine at a dosage of 10 and 20 mg/Kg/Day spent a similar amount of time (P>0.05) in the opens arms as their control wild-type littermates. The number of individuals (n) tested in three groups were I) Mice receiving regular drinking water (placebo): wild-type (n = 8) and Tg PS1/APPswe (n = 8); II) Mice receiving anatabine at a dosage of 10mg/Kg/Day: wild-type (n = 10) and Tg PS1/APPswe (n = 8) and III) Mice receiving anatabine at a dosage of 20mg/Kg/Day: wild-type (n = 10) and Tg PS1/APPswe (n = 8).