Analysis of purified RNA.

<p>(<b>A</b>) Native and (<b>B</b>) denaturing PAGE analysis of fractions collected from Superdex 75 10/300 GL gel filtration. S – concentrated RNA sample after anion exchange chromatography. (<b>C</b>) Analysis of mouse tRNA<sup>Sec</sup> by multi-angle static light scattering. The optical density at 260 nm (OD<sub>260</sub>; magenta), Rayleigh ratio (Rq; blue) and differential refractive index (RI; yellow) were monitored during analytical gel filtration on a Superdex 200 10/300 GL column. The measurement was done by Wyatt Technology Europe GmbH.</p>