Analysis of protein localization and protein levels of p120-catenin, α-catenin and E-cadherin in cells deficient of both β-catenin and plakoglobin.

A) Immunostaining of plakoglobin siRNA transfected wild type and gene disrupted cells as indicated. The cells were stained with anti p120-catenin (left column), anti α-catenin (middle column) and anti E-cadherin (right column) Abs 72 hours after siRNA transfection. Immunostaining (p120-catenin, α-catenin and E-cadherin) of untransfected and control siRNA transfected cells are included in S1 Fig. B) WB analysis of total cell lysates (10 µg TP) from untransfected (1), control siRNA (2) and plakoglobin siRNA (3) transfected wild type and gene disrupted cells as indicated. The cell lysates was prepared 72 hours after siRNA transfection and specific bands for p120-catenin (upper panel), α-catenin (middle panel) and E-cadherin (lower panel) with the corresponding actin bands are shown as indicated.