An apparent non-canonical intron in the IGS1 R non-coding RNA.

2010-08-18T01:19:32Z (GMT) by Jonathan Houseley David Tollervey
<p>A: 35 cycle RT-PCR across the apparent intron on cDNA synthesized with Superscript II and genomic DNA. cDNA was produced from a <i>trf4</i>Δ strain where this non-coding RNA is stabilized. B: Hairpin structure of IGS1 R, short homologous repeats are underlined in grey. C: 35 cycle RT-PCR across the apparent intron on <i>trf4</i>Δ cDNA synthesized using Superscript II at 42°C or Superscript III at 55°C. D: 35 cycle RT-PCR across the apparent intron on <i>trf4</i>Δ cDNA synthesized using Superscipt II or AMV. Control shows 30 cycle RT-PCR reaction across the <i>ASC1</i> mRNA intron on the same cDNA samples.</p>