Amino acid substitutes in YebN cytoplasmic regions alter <i>Xoo</i> Mn<sup>2+</sup> tolerance.

<p>The strains that <i>yebN</i> mutant (Δ<i>yebN</i>) carries extrachromosomal <i>yebN</i> wild type sequence (WT) or some point mutations in YebN cytoplasmic regions (G25A, A26N, A27N, A27ND35A and G167A) cloned into the pHM1 vector were analyzed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0021983#pone-0021983-g002" target="_blank">Figure 2A</a>. The strain that Δ<i>yebN</i> contains the pHM1 vector was the negative control (CK).</p>