Akt regulators accumulate in Salmonella induced ruffles.

HeLa cells were transfected with plasmids encoding epitope-tagged proteins as indicated. After 20 hr they were infected with Salmonella for 30 min, then fixed and processed for immunofluorescence microscopy. GFP-HA-Akt transfected cells were stained for Salmonella LPS (Cy5) and phospho Akt Ser473 (AF568: A–C), Myc (AF568: D–F) or 3×FLAG (AF568: J–L). Cells expressing PDK1-Myc (G–I) were stained for LPS (Cy5), Myc (AF488) and actin filaments (phalloidin-AF568) to reveal the ruffles.