Figure_2.tif (3.83 MB)
Adult ZAS3 knockout mice exhibit decreased number of osteoclasts.
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posted on 2013-02-20, 20:08 authored by Shujun Liu, Francesca Madiai, Kevin V. Hackshaw, Carl E. Allen, Joseph Carl, Emily Huschart, Chris Karanfilov, Alan Litsky, Christopher J. Hickey, Guido Marcucci, Sarandeep Huja, Sudha Agarwal, Jianhua Yu, Michael A. Caligiuri, Lai-Chu WuSagittal sections of femora from 1-month-old mice (A to D) or 4-month-old mice (E to H) stained with H & E (A, C, E and G) or stained for TRAP (B, D, F and H). (I) and (J) Higher magnifications of femora of 4-month-old mice stained with TRAP. Note that the number of osteoclasts (cells stained red and indicated with arrows) in WT mice (I) was more abundant than that of KO mice (J). (K) Western blot analysis of protein lysates of bone marrow stromal cells with indicated antibodies. +/+ ZAS3 WT mice, −/− ZAS3 KO mice, and +/− heterozygous ZAS3 mice.
