Adaptive immunity features from cynomolgus macaques naturally infected with <i>T</i>. <i>cruzi</i> (CH) and non-infected controls (NI).

<p>(A) The frequencies of CD3<sup>+</sup> lymphocytes and T-cell subsets (CD4<sup>+</sup> and CD8<sup>+</sup>), the expression of adhesion molecule (CD54) and activation status (CD69 and HLADR) were performed by multicolor flow cytometry. (B) The expression of cytotoxicity markers (Granzyme A, Granzyme B and Perforin) of CD8<sup>+</sup> T-cells was investigated by intracellular staining flow cytometry. (C) Analysis of B-cells, the activation status (CD69), and the expression of the regulatory FcγR (CD32) were evaluated by three-color flow cytometry. The results are expressed as mean percentage with standard error. Significant differences at <i>p<</i>0.05 are identified by (*).</p>