Ad-CXCR4 transfection protects EPCs from HG-induced dysfunction and apoptosis via activating Akt/eNOS pathways.

Representative tube formation pictures (A1–A8) and summarized data (A9) in different treatment groups. A1: Ad-null-EPCs+Con; A2: Ad-null-EPCs+Osm; A3: Ad-null-EPCs+HG; A4: Ad-CXCR4-EPCs+Con; A5: Ad-CXCR4-EPCs+Osm; A6: Ad-CXCR4-EPCs+HG; A7: Ad-CXCR4-EPCs+HG+LY294002; A8: Ad-CXCR4-EPCs+HG+L-NAME. Scale bar: 600 µm. Summarized data on migration ability (B) and the percentage of EPC apoptosis (C) in different treatment groups. *P<0.05, **P<0.01 vs. Ad-null-EPCs or Ad-null-EPCs+Osm; ++P<0.01 vs. HG+Ad-null-EPCs; #P<0.05, ##P<0.01 vs. HG+Ad-CXCR4-EPCs; §P<0.05 vs. HG+Ad-CXCR4-EPCs+LY294002, n = 6/group. Con: control (basal medium); Osm: osmotic control; HG: high glucose medium; Ad-null-EPCs: EPCs transfected with Ad-null; Ad-CXCR4-EPCs: EPCs transfected with Ad-CXCR4.