Activation of antigen-nonspecific immune responses by CpG-ODN developed for rabbits.

Rabbit splenocytes were stimulated with 2µM of different CpG-ODN. (A) Induction of cytokines was analyzed by RT-PCR. (B) Production of IgM was determined by ELISA. (C) Proliferation of splenocytes was measured by MTS assay. Data shown represent mean ± SD (n = 3 independent experiments).*P<0.05 vs. the induction by CpG-C4609.