Activation of BMDC by GB.

Bone marrow cells (1 X 10⁶) were incubated with 50 ng/ml GM-CSF and 50 ng/ml IL-4 for 6 days, and then stimulated with GB or GR for 24 hours. (A) Morphological changes were analyzed by microscopy. (B) Expression of surface co-stimulatory molecules measured by flow cytometry. (C) CD86 and MHC class II expression levels were measured from GR- or GB-treated BMDC in indicated dose. All data are representative of or the average of analyses of 6 independent samples (2 samples per experiment, total 3 independent experiments). *p < 0.05, **p < 0.01.