Accumulation of ubiquitin in mutant FUS transgenic rats.

<p>(A–L) Double-fluorescence staining revealed accumulation of ubiquitin in the cortex (D–F) and spinal cord (J–L) of the mutant FUS transgenic rats (line 16) at paralysis stages, but not in tissues of the age-matched normal FUS transgenic rats (line 20) (A–C: cortex; G–I: spinal cord). Coronal sections of the frontal cortex and traverse sections of the lumbar cord were immunostained with antibodies to ubiquitin (green) and human FUS (red). Scale bars: A–F, 20 µm.</p>