Ability of ApHMW1C to complement a deficiency in HMW1C.

<p>Panel <b>A</b> shows Western immunoblots of whole cell sonicates of <i>E. coli</i> BL21(DE3)/pACYC-HMW1ΔC (lane 1), <i>E. coli</i> BL21(DE3)/pACYC-HMW1ΔC + pET45b-HMW1C (lane 2), and <i>E. coli</i> BL21(DE3)/pACYC-HMW1ΔC + pET45b-ApHMW1C (lane 3). Lane 1 contains twice as much protein as loaded in lanes 2 and 3 to increase the visibility of the non-glycosylated HMW1 species. The blot in the upper panel was performed with a guinea pig antiserum reactive with HMW1, and the blot in the lower panel was performed with a guinea pig antiserum reactive with <i>H. influenzae</i> HMW1C. The asterisk indicates the glycosylated HMW1 pro-protein, and the plus sign indicates the non-glycosylated HMW1 pro-protein. The diamond indicates the glycosylated HMW1 mature protein, and the circle indicates the non-glycosylated HMW1 mature protein. Panel <b>B</b> shows <i>in vitro</i> adherence results comparing adherence by <i>E. coli</i> BL21(DE3)/pACYC-HMW1ΔC (<i>hmw1AB</i>), <i>E. coli</i> BL21(DE3)/pACYC-HMW1ΔC + pET45b-HMW1C (<i>hmw1AB</i> + <i>hmw1C</i>), and <i>E. coli</i> BL21(DE3)/pACYC-HMW1ΔC + pET45b-ApHMW1C (<i>hmw1AB</i> + <i>Aphmw1C</i>) to Chang epithelial cells. Bars and error bars represent mean and standard error measurements from a representative assay with measurements performed in triplicate.</p>