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(A) Surface expression of CXCR2 on GR-1–positive leukocytes of ST3Gal-IV and WT mice was analyzed by flow cytometry
figure
posted on 2011-12-31, 18:35 authored by David Frommhold, Andreas Ludwig, M. Gabriele Bixel, Alexander Zarbock, Inna Babushkina, Melitta Weissinger, Sandra Cauwenberghs, Lesley G. Ellies, Jamey D. Marth, Annette G. Beck-Sickinger, Michael Sixt, Bärbel Lange-Sperandio, Alma Zernecke, Ernst Brandt, Christian Weber, Dietmar Vestweber, Klaus Ley, Markus SperandioLeukocytes were incubated with an allophycocyanin-labeled GR-1 mAb, a PE-labeled anti-CXCR2 mAb, and a PE-labeled isotype control. (B) For ligand binding studies, leukocytes from the peripheral blood of WT ( = 4), ST3Gal-IV ( = 3), and CXCR2 ( = 3) mice were incubated with different dilutions of CF-CXCL8 and subsequently analyzed for bound fluorescence by flow cytometry. Peripheral blood leukocytes from WT mice were treated with 100 mU/ml neuraminidase for 60 min and investigated for CF-CXCL8 binding. Data are given as mean ± SD. P < 0.05 for WT versus ST3Gal-IV, CXCR2, and WT + Neu for 30 and 90 nM CF-CXCL8, respectively. MFI, mean fluorescence intensity.
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Taken from "Sialyltransferase ST3Gal-IV controls CXCR2-mediated firm leukocyte arrest during inflammation"
The Journal of Experimental Medicine 2008;205(6):1435-1446.
Published online 9 Jun 2008
PMCID:PMC2413039.
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