AQP2 and p-AQP2 IMCD localization.

Immunoperoxidase microscopy of AQP2 and p-AQP2 in the inner medulla. Sections were incubated with affinity-purified anti-AQP2 (A – E) or affinity-purified anti-p-AQP2 antibody (F – J), and labeling was visualized using peroxidase-conjugated secondary antibody. A – B: In standard salt diet Sham rats AQP2 and p-AQP2 labeling is present at the apical and intracellular domains (arrows). C – F: AQP2 and p-AQP2 stainings from L-Sham and L-Sham+d rats were mainly situated in apical domains with weak intracellular labeling. G – L: In contrast, all HF groups demonstrated strong apical immunoperoxidase labeling of AQP2 and p-AQP2 with virtually no staining in intracellular domains. Magnification x 630.