APAP-induced apoptosis.

Apoptosis in J774.2 macrophages and HepG2 cells were measured after APAP and DAS treatment using Flow cytometry as described in the vendor’s protocol using the Becton Dickinson FACSCantoII analyzer. Apoptotic cells were estimated by the percentage of cells that stained positive for Annexin V-FITC. Representative histograms of flow cytometric results are shown.