Video S1 - ALS-Associated TDP-43 Induces Endoplasmic Reticulum Stress, Which Drives Cytoplasmic TDP-43 Accumulation and Stress Granule Formation
Association of 218–414 TDP-43-mCherry inclusions with the Golgi apparatus. Neuro2a cells were transfected with the 218–414 TDP-43-mCherry construct, and treated with 10 µM MG132 for 24 h starting at 24 h post-transfection. Cells were processed for immunocytochemistry against GM130 (green) and serial z-stack images were taken at 0.50 µm intervals using confocal microscopy and post-processed using Olympus Fluoview v2.0 software. mCherry fluorescence is shown in red and nuclei (Hoechst dye) are shown in blue. Scale bar represents 10 µm.