Figure_11.tif (133.03 kB)
AF4-MALS analysis of BSA (1 mg/ml) at 25°C (0.1 M phosphate buffer, pH 7.0).
figure
posted on 2013-09-10, 01:30 authored by Vera A. Borzova, Kira A. Markossian, Dmitriy A. Kara, Natalia A. Chebotareva, Valentina F. Makeeva, Nikolay B. Poliansky, Konstantin O. Muranov, Boris I. KurganovMolar mass versus elution time plot (points) is overlaid on the UV detector fractogram (solid line). A4F conditions: axial (detector) flow 1 ml/min, focus flow 5 ml/min, cross flow 5 ml/min for 14 min and then linear decay to 0.1 ml/min within 20 min plus 8 min at 0 ml/min.