AF4-MALS analysis of BSA (1 mg/ml) at 25°C (0.1 M phosphate buffer, pH 7.0).

<p>Molar mass versus elution time plot (points) is overlaid on the UV detector fractogram (solid line). A4F conditions: axial (detector) flow 1 ml/min, focus flow 5 ml/min, cross flow 5 ml/min for 14 min and then linear decay to 0.1 ml/min within 20 min plus 8 min at 0 ml/min.</p>