AE-HPLC-PCR Quantification of p-HDL in Control (AA) and SCD Plasma.

(A) DCF chromatogram showing relative levels of DCF fluorescence (index of oxidizability) for p-HDL in control and SCD plasma. At 30–44 minutes, DCF fluorescence intensity in separated HDL in SCD plasma (black line) is greater than in control plasma (gray line). (B) Dividing DCF fluorescence intensity under these peaks by the subject’s plasma apoA-I concentration (µg/mL) yields relative fluorescence units (RFU) per apoA-I (µg/mL). P-HDL are increased in SCD subjects compared to control subjects (** = p<0.01, n = 6).