γδTCR/CD3 complex expression and rearrangements in human peripheral blood eosinophils.

<p>(A) Real time PCR analysis of expression of individual chains from γδTCR/CD3 complex in eosinophils, γδ T cells and Colo-205 cells. Average expression in Colo-205 cells is chosen as reference (samples were analysed in triplicate using primers listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005926#pone.0005926.s005" target="_blank">Table S1</a>). (B) TCRγ and δ rearrangements in peripheral blood eosinophils analysed by RT-PCR using primers listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005926#pone.0005926.s006" target="_blank">Table S2</a>. CD8 amplification is performed to exclude lymphocyte contamination. Colo-205 are chosen as negative control (C) Spectratyping analysis of CDR3 length profiles for Vγ9-Jγ1/2 ; Vγ9-JγP and Vδ2-Cδ families in eosinophils and in γδ T cells from the same donor. (D) Expression of rearranged γ and δTCR at the single cell level in peripheral blood eosinophils and γδT cells detected by <i>in situ</i> hybridization using anti-sense and control sense probes specific for Vδ2-Cδ, VγI-JγP and for Vδ1-Jδ4 used as negative control.</p>