5hmC was involved in DNA demethylation in fused cells.

(A) RT-qPCR analysis of the gene expression of Tet enzymes (mean + s.d.; *P<0.05, ** P<0.01, n = 3). (B) Immunoblotting analysis of 5hmC of genomic DNA samples from NIH3T3 cells, mESCs and fused cells at day 3. (C) Immunostaining analysis of 5hmC in NIH3T3 cells, mESCs and fused cells at day 3. Scale bar, 100 μm. (D) (E) Detection of 5hmC and 5mC at OCT4 promoter by glucMS-qPCR (as a percentage of total cytosine). Results were shown as means and s.d. (n = 3). D1, D2 and D3 indicated fused cells on day 1, 2 and 3, respectively.