σ<sup>54</sup> binds conserved sites in <i>Salmonella enterica</i>.

<p>Targeted validation of predicted σ<sup>54</sup> binding sites in <i>S</i>. <i>enterica</i>. Enrichment (occupancy units) at predicted σ<sup>54</sup> binding sites in <i>S</i>. <i>enterica</i> was measured by ChIP-qPCR with anti-σ<sup>54</sup> (black bars) or no antibody (white bars). The cognate promoter IDs (Tables <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005552#pgen.1005552.t001" target="_blank">1</a> & <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005552#pgen.1005552.t002" target="_blank">2</a>) are indicated in parentheses. Error bars represent the standard deviation from three independent biological replicates. Significant differences between wild-type and Δ<i>rpoN</i> values are indicated (*<i>p</i> ≤ 0.05, **<i>p</i> ≤ 0.01, ***<i>p</i> ≤ 0.001). Note that the <i>y</i>-axis scale differs for first five regions indicated. Underlined genes indicate σ<sup>54</sup> binding sites previously identified by [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005552#pgen.1005552.ref014" target="_blank">14</a>].</p>