3′ UTR <i>SLC11A1</i> gene polymorphism analysis in resistant and susceptible macrophages.

<p>Representative results from resistant (R) and susceptible (S) cattle genomic DNA single-stranded conformational analysis (SSCA) indicating the homozygous GT13 (175 bp) and heterozygous GT13/GT14 (177 bp) alleles. DNA from cattle genetically characterized as susceptible (SLC11A1s) or resistant (SLC11A1r) to standardized <i>Brucella abortus</i> in vivo challenge were used as controls (Cx) (A). DNA sequencing of PCR amplicons of <i>SLC11A1</i> 3′UTR confirmed the SSCA results evidencing the only one heterozygous genotype among all phenotyped susceptible and resistant cattle to BCG (B).</p>