Figure_2.tif (576.77 kB)
3′ UTR SLC11A1 gene polymorphism analysis in resistant and susceptible macrophages.
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posted on 2013-05-15, 07:34 authored by Hugo Esquivel-Solís, Antonio J. Vallecillo, Alejandro Benítez-Guzmán, L. Garry Adams, Yolanda López-Vidal, José A. Gutiérrez-PabelloRepresentative results from resistant (R) and susceptible (S) cattle genomic DNA single-stranded conformational analysis (SSCA) indicating the homozygous GT13 (175 bp) and heterozygous GT13/GT14 (177 bp) alleles. DNA from cattle genetically characterized as susceptible (SLC11A1s) or resistant (SLC11A1r) to standardized Brucella abortus in vivo challenge were used as controls (Cx) (A). DNA sequencing of PCR amplicons of SLC11A1 3′UTR confirmed the SSCA results evidencing the only one heterozygous genotype among all phenotyped susceptible and resistant cattle to BCG (B).
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BiochemistryNeurochemistryNeurochemicalsNitric oxideEvolutionary biologypopulation geneticsGenetic polymorphismgeneticsimmunologyImmune cellsmonocytesimmunityInnate immunityneuroscienceVeterinary diseasesZoonotic diseasesBovine tuberculosisVeterinary medicineVeterinary immunologyutrpolymorphismresistantsusceptible
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