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2-DE image of 10 µL human serum and mass spectra of glycopeptides of 2-DE separated α1-antitrypsin.

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posted on 2013-02-20, 22:09 authored by Piliang Hao, Yan Ren, Yongming Xie

(A) 2-DE image of 10 µL human serum. Two largest spots of α1-antitrypsin were chosen for glycosylation analysis and named as A1PI-1 and A1PI-2. (B)–(C): Mass spectra of the glycopeptides of A1PI-1 and A1PI-2 in the mass range of 5500-7000 Dalton. Data were shown in average value. Compared with A1PI-1, the relative abundance of diantennary N-glycans indicated by arrows in A1PI-2 increased significantly, while the triantennary N-glycans decreased significantly.

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