2D-DIGE image of proteins of sarkosyl-insoluble aggregates isolated from the GT81-1C [<i>PSI</i><sup>+</sup>][<i>PIN</i><sup>+</sup>] strain and its [<i>psi</i><sup>−</sup>][<i>pin</i><sup>−</sup>] derivative (A).

<p>Spots corresponding to proteins from [<i>PSI</i><sup>+</sup>][<i>PIN</i><sup>+</sup>] lysate and the [<i>psi<sup>−</sup></i>][<i>pin</i><sup>−</sup>] control are red and green, respectively, while yellow spots correspond to proteins present in both compared samples. SDS-PAGE image of proteins from the same DRAFs that were insoluble in UTC buffer (B). Proteins identified by mass-spectrometry are indicated. Mr – protein molecular mass markers. Identification data are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116003#pone-0116003-t004" target="_blank">Table 4</a>; mass spectra are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116003#pone.0116003.s004" target="_blank">S4 Table</a>.</p>